To rail against nature is folly. All things must accept an end.
Vespa was the queen of the Hive, a tribe that is walled off from the lands of the Pale King. The Hive rejected Hallownest's attempt at perpetuation, arguing that it is pointless to go against nature. Vespa eventually grew so large that she could no longer leave the Hive.
After Hive Knight, Vespa's former protector, is defeated and the Hiveblood Charm is acquired, Vespa's spirit can be found in the same area that Hive Knight is fought. Her corpse can be seen in this room in the background.
She tells the Knight that she knows their kind, but that the pale beings, the Pale King and the White Lady, are responsible for the Knight's nature. Like all other spirits, the Dream Nail can be used on her, giving 1 Essence.
Hive Queen Vespa is located in the northeast part of the Hive.